It is possible to use the Ferrovie del Sud Est railway service, by taking a train leaving from Bari and getting to the Alberobello Train Station in approximately one hour and thirty minutes.
Driving along the A14 motorway, reach Bari and then continue on the SS 16 towards Brindisi-Lecce, until the exit for Monopoli. At this point, simply follow the signs for Alberobello. Alternatively, you can take the SS 100 towards Taranto and exit at Casamassima, and then continue towards Turi then Putignano, then finally Alberobello.
The nearest airports are those of Bari-Palese, 65 km and Brindisi-Casale, 80 km.